As previously mentioned, in 1994, biologists at the Department of Plant Biology, Louisiana State University performed extensive testing of internal fluid and gas pressure in plants.
They found that an aquatic plant’s internal CO2 pressure is about 3.5X greater than the CO2 pressure in the surrounding water.
The CO2 solution used for performing Reverse Respiration however, is much higher pressure than the normal atmospheric CO2 and that of the plant. The unopened container of the CO2 solution has some 17X more pressure than the natural atmospheric CO2 pressure, but almost immediately stabilizes at about 3X the normal atmospheric CO2 pressure upon opening, where it remains on average for approximately 72 hours, if undisturbed.